Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal

• Supplier: Abcam
• Catalog: ab177954
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR12716
• Applications: WB FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Natriuretic Peptide Receptor C aa 500 to the C-terminus (Cysteine residue)
• Description: Rabbit Monoclonal
• Gene: NPR3
• Conjugate: Unconjugated

Product images

All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (purified)Lane 1 : Human tissue lysateLane 2 : Human tissue lysateLysates/proteins at 1/10 dilution per lane.SecondaryHRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/7000 dilution (purified)Lane 1 : Mouse kidney tissue lysateLane 2 : Rat kidney tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)Lane 1 : Mouse kidney tissue lysateLane 2 : Rat kidney tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab177954 at a dilution of 1 in 270 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)Lane 1 : Human fetal kidney lysateLane 2 : Human placenta lysateLane 3 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Flow Cytometric analysis of permeabilized HeLa cells labeling Natriuretic Peptide Receptor C with unpurified ab177954 at 1/100 dilution (red) or a rabbit IgG (negative) (green).