Anti-NCAM antibody [123C3]
| Name | Anti-NCAM antibody [123C3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab9272 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 123C3 |
| Applications | ICC/IF IHC-F IHC-P WB IHC-P FC |
| Species Reactivities | Rat, Human, Zebrafish |
| Antigen | This antibody was raised against a small cell lung carcinoma specimen |
| Description | Mouse Monoclonal |
| Gene | NCAM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![ab9272 at a dilution of 1/1000, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing hippocampal neuron body and processes: [A] 20x objective and [B] punctate cytoplasmic staining; 40x objective. No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27870_ab9272.jpg)
ab9272 at a dilution of 1/1000, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing hippocampal neuron body and processes: [A] 20x objective and [B] punctate cytoplasmic staining; 40x objective. No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Immunofluorescence staining images of 9 day old zebrafish embryos. ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
Immunofluorescence staining images of 9 day old zebrafish embryos. ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
Immunofluorescence staining images of 9 day old zebrafish embryos. ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
Immunostaining of the neural cell adhesion molecule (NCAM) in small cell lung cancer cell line NCI-H82
![Overlay histogram showing SH-SY5Y cells stained with ab9272 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9272, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27875_NCAM-Primary-antibodies-ab9272-28.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab9272 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9272, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Selective expansion of the beta-cell compartment in the pancreas of keratinocyte - Selective expansion of the beta-cell compartment in the pancreas of keratinocyte
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A peptide from the first fibronectin domain of NCAM acts as an inverse agonist - A peptide from the first fibronectin domain of NCAM acts as an inverse agonist
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Prognostic factors in resected non-small cell lung cancer: an immunohistochemical - Prognostic factors in resected non-small cell lung cancer: an immunohistochemical
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Radioimmunotherapy of small-cell lung cancer xenografts using 131I-labelled - Radioimmunotherapy of small-cell lung cancer xenografts using 131I-labelled
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Hot spots of antigenicity in the neural cell adhesion molecule NCAM. - Hot spots of antigenicity in the neural cell adhesion molecule NCAM.
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Neural cell adhesion molecule expression, neuroendocrine differentiation and - Neural cell adhesion molecule expression, neuroendocrine differentiation and
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Expression of neural cell adhesion molecule-related sialoglycoprotein in small - Expression of neural cell adhesion molecule-related sialoglycoprotein in small
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Monoclonal antibody 123C3, identifying small cell carcinoma phenotype in lung - Monoclonal antibody 123C3, identifying small cell carcinoma phenotype in lung
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Monoclonal antibody 123C3 in lung tumour classification. Immunohistology of 358 - Monoclonal antibody 123C3 in lung tumour classification. Immunohistology of 358
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