All lanes : Anti-NDUFA10 antibody [EPR12135] (ab174829) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : Raji cell lysateLane 5 : Human fetal heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling NDUFA10 with ab174829 at 1/50 dilution.
Immunofluorescent analysis of HeLa cells, labeling NDUFA10 with ab174829 at 1/50 dilution (red). DAPI nuclear staining (blue).
Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal heart lysate, or 2) 1x PBS (negative control); labeling NDUFA10, immunoprecipitated using ab174829 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.