All lanes : Anti-NDUFA8 antibody [EPR15303] (ab184952) at 1/20000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal muscle lysateLane 3 : MCF7 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of NDUFA8 in Human fetal heart lysate immunoprecipitated with ab184952 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal heart lysate.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.