Anti-NDUFA9 antibody
| Name | Anti-NDUFA9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55521 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: RVFEISPFEP WITRDKVERM HITDMKLPHL PGLEDLGIQA TPLELKAIEV LRRHRTYRWL SAEIEDVKPA KTVNI, corresponding to amino acids 303-378 of Human NDUFA9 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | NDUFA9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
NDUFA9 antibody (ab55521) used in immunohistochemistry at 8ug/ml on formalin fixed and paraffin embedded human small Intestine.
ab55521 at 10 ug/ml staining NDUFA9 in mouse NIH/3T3 cells by Immunocytochemistry/ Immunofluorescence.
![Overlay histogram showing HepG2 cells stained with ab55521 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55521, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/28364_NDUFA9-Primary-antibodies-ab55521-2.jpg)
Overlay histogram showing HepG2 cells stained with ab55521 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55521, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.