Anti-NDUFB4 antibody [EPR16240] (ab192243) at 1/20000 dilution + Human fetal heart lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB4 with ab192243 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.(inset: negative control).
All lanes : Anti-NDUFB4 antibody [EPR16240] (ab192243) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Human cerebellum lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-NDUFB4 antibody [EPR16240] (ab192243) at 1/5000 dilution + Rat kidney lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling NDUFB4 with ab192243 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.(inset: negative control).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A549 cells labeling NDUFB4 with ab192243 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).The two negative controls are anti-NDUFB4 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.