All lanes : Anti-NDUFB7 antibody [EPR15903] (ab188575) at 1/1000 dilutionLane 1 : PC3 cell lysateLane 2 : HL60 cell lysateLane 3 : A431 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human papillary adenocarcinoma of thyroid tissue labelling NDUFB7 with ab188575 at a 1/100 dilution. Counter stained with hematoxylin.
Immunofluorescence analysis of, 4% paraformaldehyde-fixed, HeLa cells labelling NDUFB7 with ab188575 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
Flow cytometry analysis of 2% paraformaldehyde-fixed PC3 cells labelling NDUFB7 with ab188575 at a 1/340 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
Western blot analysis on immunoprecipitation pellet from A431 cell lysate labelling NDUFB7 with ab188575 at 1/50 dilution and goat anti-rabbit IgG, (H+L), peroxidase conjugated at a 1/1000 dilution.