All lanes : Anti-NDUFB9 antibody [EPR15955] (ab188581) at 1/10000 dilutionLane 1 : Human fetal liver lysateLane 2 : HeLa cell lysateLane 3 : 293 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB9 with ab188581 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling NDUFB9 with ab188581 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling NDUFB9 with ab188581 at 1/440 dilution compared to a Rabbit monoclonal IgG isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of NDUFB9 immunoprecipitated from Human fetal liver lysate using ab188581 at 1/50 (Lane 1). Lane 2: PBS instead of Human fetal liver lysates.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.