Anti-NDUFB9 antibody [EPR15955-78]

• Supplier: Abcam
• Catalog: ab200198
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15955-78
• Applications: WB IP ICC/IF ICC/IF FC IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human NDUFB9 aa 50 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: NDUFB9
• Conjugate: Unconjugated

Product images

All lanes : Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/5000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysateLane 2 : HEK293 (Human embryonic kidney) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/5000 dilution + Human fetal liver lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/1000 dilutionLane 1 : Mouse kidney lysateLane 2 : Mouse spleen lysateLane 3 : Rat heart lysateLane 4 : Rat spleen lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Cytoplasm staining on Human kidney tissue is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Cytoplasm staining on mouse cardiac muscle tissue is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Cytoplasm staining on Jurkat cell line is observed.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab200198 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NDUFB9 with ab200198 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

NDUFB9 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200198 at 1/150 dilution.Western blot was performed from the immunoprecipitate using ab200198 at 1/1000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Jurkat whole cell lysate 10 µg (Input).Lane 2: ab200198 IP in Jurkat whole cell elysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200198 in Jurkat whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.