All lanes : Anti-NDUFC2 antibody [EPR16499] (ab192265) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFC2 with ab192265 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde-fixed A549 cells labeling NDUFC2 with ab192265 at 1/30 dilution (pink) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.