All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : 293T cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse kidney lysateLane 3 : Mouse spleen lysateLane 4 : Rat brain lysateLane 5 : Rat kidney lysateLane 6 : C6 cell lysateLane 7 : RAW264.7 cell lysateLane 8 : PC-12 cell lysateLane 9 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
Immunohistochemical analysis of paraffin-embedded rat stomach tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
Lane 1: 293T cell lysate was immunoprecipitated using ab192022 at a 1/40 dilution. Secondary used was anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.Lane 2: PBS instead of 293T lysates.