Immunofluorescence analysis of 293 cells labeling NDUFS3 with ab177471 at 1/50 dilution (green). DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from (1) fetal heart lysate or (2) 1XPBS (negative control) using ab177471at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemcal analysis of paraffin-embedded Human heart tissue labeling NDUFS3 with ab177471 at 1/50 dilution.
Immunohistochemcal analysis of paraffin-embedded Human kidney tissue labeling NDUFS3 with ab177471 at 1/50 dilution.
All lanes : Anti-NDUFS3 antibody [EPR12782] - C-terminal (ab177471) at 1/1000 dilutionLane 1 : HepG2 lysateLane 2 : 293T lysateLane 3 : HL60 lysateLane 4 : Fetal heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique