Flow cytometric analysis of NIH 3T3 cells (paraformaldehyde-fixed, 2%) labeling NDUFS6 with ab195808 at 1/90 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).
Immunofluorescent analysis of HeLa cells (100% methanol-fixed, 0.1% tritonX-100 permeabilized) labeling NDUFS6 with ab195808 at 1/200 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).Note: Cytoplasm staining on HeLa cell line was observed.
Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling NDUFS6 with ab195808 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cytoplasm staining on human squamous cell carcinoma of cervix tissue was observed.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFS6 with ab195808 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cytoplasm staining on human kidney tissue was observed.
Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/1000 dilution + PC12 cell lysateSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Rat brain lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/10000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human skeletal muscle lysateBlocking peptides at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/10000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal brain lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilutiondeveloped using the ECL technique