All lanes : Anti-NDUFV2 antibody [EPR15350(B)] (ab183717) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : Ramos cell lysateLane 3 : Jurkat cell lysateLane 4 : A549 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human endometrial adenocarcinoma tissue labeling NDUFV2 with ab183717 at a 1/100 dilution. Counter stained with hematoxylin.
Western blot analysis on immunoprecipitation from 1) A549 cell lysate, or 2) TBS, labeling NDUFV2 using ab183717 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.