All lanes : Anti-NDUFV2 antibody [EPR15351(B)] (ab183715) at 1/1000 dilutionLane 1 : A549 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : Ramos cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Anti-NDUFV2 antibody [EPR15351(B)] (ab183715) at 1/20000 dilution + Human fetal heart lysate at 20 µgSecondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human hepatocellular carcinoma tissue labeling NDUFV2 with ab183715 at a 1/500 dilution. Counter stained with hematoxylin.
Immunofluorescence analysis of, acetone-fixed, HeLa cells labeling NDUFV2 with ab183715 at a 1/100 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
Western blot analysis on immunoprecipitation from 1) Human fetal heart lysate, or 2) TBS, labeling NDUFV2 using ab183715 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.