All lanes : Anti-NECAB1 antibody [4G8] (ab118411) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY NECAB1 cDNA Lysates/proteins at 5 µg per lane.
HEK293T cells transfected with either pCMV6-ENTRY overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab118411 at 1/100 dilution, and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using ab118411 at 1/100 dilution,(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using ab118411 at 1/100 dilution,(Red), compared to a nonspecific negative control antibody,(Blue).