Anti-NEDD8 antibody [Y297]

• Supplier: Abcam
• Catalog: ab81264
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: Y297
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Rat, Human
• Antigen: Synthetic peptide corresponding to residues in the N terminal of human NEDD8
• Description: Rabbit Monoclonal
• Gene: NEDD8
• Conjugate: Unconjugated

Product images

All lanes : Anti-NEDD8 antibody [Y297] (ab81264) at 1/1000 dilutionLane 1 : C6 Cell LysateLane 2 : Raw264.7 Cell LysateLane 3 : PC-12 Cell LysateLane 4 : 3T3 Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-NEDD8 antibody [Y297] (ab81264) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate using ab81264 at 1/500 dilution. Band at 90 kDa shows Neddylated cullins, 50 kDa the IgG fraaction and 9 kDa Free NEDD8.

ab81264, at 1/100 dilution, staining NEDD8 in human lung carcinoma tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded section.

Product References

The Nedd8-activating enzyme inhibitor MLN4924 thwarts microenvironment-driven - The Nedd8-activating enzyme inhibitor MLN4924 thwarts microenvironment-driven

Godbersen JC, Humphries LA, Danilova OV, Kebbekus PE, Brown JR, Eastman A, Danilov AV. Clin Cancer Res. 2014 Mar 15;20(6):1576-89.

The transient nature of Bunyamwera orthobunyavirus NSs protein expression: - The transient nature of Bunyamwera orthobunyavirus NSs protein expression:

van Knippenberg I, Fragkoudis R, Elliott RM. PLoS One. 2013 May 8;8(5):e64137.