All lanes : Anti-NELL2 antibody (ab80885) at 1 µg/mlLane 1 : Mouse Hippocampus Tissue Lysate Lane 2 : Mouse Cerebellum Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-NELL2 antibody (ab80885) at 1 µg/mlLane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell LysateLane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 3 : SK N BE (Human neuroblastoma) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of NELL2 staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80885, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.