Anti-Nesprin 1 antibody [EPR14196]

• Supplier: Abcam
• Catalog: ab192234
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14196
• Applications: ICC/IF ICC/IF WB IHC-P FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 8250-8350
• Description: Rabbit Monoclonal
• Gene: SYNE1
• Conjugate: Unconjugated

Product images

Flow cytometric analysis of HACAT cells (paraformaldehyde-fixed, 2%) labeling Nesprin 1 with ab192234 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunofluorescent analysis of A673 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Nesprin 1 with ab192234 at 1/50 dilution (4.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution; counter-stained with DAPI (blue).Negative controls: anti-Nesprin 1 at 1/50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Nesprin 1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Nesprin 1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Nesprin 1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

All lanes : Anti-Nesprin 1 antibody [EPR14196] (ab192234) at 1/1000 dilutionLane 1 : RAW 264.7 cell lysateLane 2 : PC12 cell lysateLane 3 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-Nesprin 1 antibody [EPR14196] (ab192234) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : HACAT cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution