All lanes : Anti-NETO2 antibody - N-terminal (ab171651) at 1 µg/mlLane 1 : Rat whole brain lysate (P31) at 25 µgLane 2 : Mouse whole brain lysate (3mnths) at 25 µgLane 3 : HEK293 transfected with HA-Neto1 plasmid at 25 µgLane 4 : HEK293 transfected with myc-Neto2 plasmid at 25 µlLane 5 : non-transfected HEK293 at 25 µgSecondaryHRP conjugated goat anti-rabbit at 1/8000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-NETO2 antibody - N-terminal (ab171651) at 1/100 dilution + Jurkat cell line lysate at 35 µgdeveloped using the ECL technique
Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling NETO2 with ab171651 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow Cytometric analysis of Jurkat cells with ab171651 antibody at a dilution of 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated Donkey-anti-Rabbit secondary antibodies were used for the analysis.