Anti-Neurexin II alpha antibody
| Name | Anti-Neurexin II alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab34245 |
| Prices | $242.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Rat Neurexin II alpha |
| Description | Rabbit Polyclonal |
| Gene | NRXN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
(Image courtesy of Human Protein Atlas)ab34245 staining Neurexin II alpha protein in normal adult human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab34245 (1/700 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6..
Anti-Neurexin II alpha antibody (ab34245) at 1/400 dilution + whole cell lysate prepared from murine N2A cells at 20 µgSecondaryGoat polyclonal conjugated to Dylight 800 at 1/20000 dilutionPerformed under reducing conditions.
ICC/IF image of ab34245 stained SKNSH cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34245, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Diurnal rhythms in neurexins transcripts and inhibitory/excitatory synapse - Diurnal rhythms in neurexins transcripts and inhibitory/excitatory synapse
Shapiro-Reznik M, Jilg A, Lerner H, Earnest DJ, Zisapel N. PLoS One. 2012;7(5):e37894.
Subcellular distribution and expression of prenylated Rab acceptor 1 domain - Subcellular distribution and expression of prenylated Rab acceptor 1 domain
Borsics T, Lundberg E, Geerts D, Koomoa DL, Koster J, Wester K, Bachmann AS. Cancer Sci. 2010 Jul;101(7):1624-31.