Anti-Neurofascin antibody
| Name | Anti-Neurofascin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31457 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P IHC-F |
| Species Reactivities | Mouse, Rat, Human, Marmoset |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Mouse Neurofascin |
| Description | Rabbit Polyclonal |
| Gene | NFASC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Neurofascin antibody (ab31457) at 1 µg/ml + Brain(Mouse)Whole Cell Lysate at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab31457 stained human SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31475, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Ab31457 used on Rat cerebellum, Purkinje cells are variously positive: there is a clear punctate positivity of secondary dendritic processes, a strong membraneous positivity of the somata and also a single strongly positive fibre that seemingly enters the Granule Cell Layer indicated by red arrowheads axons of P.cells. The Glomeruli of the GCL are moderately positive. There is also a linear pattern of positivity within the GCL that may well be fibres.See Abreview
Immunohistochemistical detection of Neurofascin using antibody ab31457 on PFA perfusion-fixed rat brain sections. Primary antibody ab31457 was used at 1/1000 incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. Some cytoplasmic staining was observed in granular neurons and neuronal processes (arrows on the figure on the right). The lefthand figure shows the hippocampal granular cell staining (objective X20), the righthand figure shows this at higher magnification. This staining is consistent with published observation of cytoplasmic localisation of Neurofascin in the cytoplasm of hippocampal cells and their axon initial segment (Burkarth N MCN 2007).
Product References
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Bremer J, Baumann F, Tiberi C, Wessig C, Fischer H, Schwarz P, Steele AD, Toyka KV, Nave KA, Weis J, Aguzzi A. Nat Neurosci. 2010 Mar;13(3):310-8.
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Lovato L, Cianti R, Gini B, Marconi S, Bianchi L, Armini A, Anghileri E, Locatelli F, Paoletti F, Franciotta D, Bini L, Bonetti B. Mol Cell Proteomics. 2008 Dec;7(12):2337-49.