Anti-Neurogenin3 antibody
| Name | Anti-Neurogenin3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38548 |
| Prices | $385.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to Human Neurogenin3 aa 40-69 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | NEUROG3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Neurogenin3 antibody (ab38548) at 1/1000 dilution + Mouse liver tissue lysateSecondaryHRP goat anti-rabbit (H+L) at 1/5000 dilution
ab38548 (4µg/ml) staining Neurogenin-3 in human jejunum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human large intestine tissue labelling Neurogenin3 with ab38548. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.
ab38548 staining Neurogenin3 in mouse psotnatal day 8 testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4$ paraformaldehyde and blocked with 5% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in 0.1M citric acid/sodium citrate. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A FITC-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
Immunocytochemistry/Immunofluorescence analysis of NCI-H460 cells labelling Neurogenin3 (green) with ab38548. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstained with DAPI (blue)
Product References
Neonatal diabetes and congenital malabsorptive diarrhea attributable to a novel - Neonatal diabetes and congenital malabsorptive diarrhea attributable to a novel
Pinney SE, Oliver-Krasinski J, Ernst L, Hughes N, Patel P, Stoffers DA, Russo P, De Leon DD. J Clin Endocrinol Metab. 2011 Jul;96(7):1960-5.
Gene expression profiling of a mouse model of pancreatic islet dysmorphogenesis. - Gene expression profiling of a mouse model of pancreatic islet dysmorphogenesis.
Wilding Crawford L, Tweedie Ables E, Oh YA, Boone B, Levy S, Gannon M. PLoS One. 2008 Feb 20;3(2):e1611.