Anti-Neuronatin antibody

• Supplier: Abcam
• Catalog: ab27266
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-P IP
• Species Reactivities: Mouse, Human, Rat
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse Neuronatin
• Description: Rabbit Polyclonal
• Gene: NNAT
• Conjugate: Unconjugated

Product images

Anti-Neuronatin antibody (ab27266) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 20 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-Neuronatin antibody (ab27266) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (blocked with 3% Milk) at 50 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ab27266 at 1/1000 staining human pituitary cells by IHC-P The antibody was tested on normal human pituitary slide sections (formalin fixed/paraffin embedded). This gave good staining over a range of concentrations with a microwave/pressure antigen retrieval step. Staining was strongly associated with the cell membrane and blocked by incubating with the neuronatin antibody with the peptide which the antibody was raised against.See Abreview

Anti-Neuronatin antibody (ab27266) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

ICC/IF image of ab27266 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemical analysis of PFA-perfusion fixed mouse brain tissue, staining Neuronatin with ab27266. Tissue was post-fixed in 4% paraformaldehyde overnight at 4°C. Sections were incubated in a blocking buffer of 0.3% Triton-X-100, 1% BSA and 10% NGS in PBS for 2 hours at room temperature, before incubating with primary antibody (1/100) for 2 days at 4°C. An AlexaFluor®-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.

Product References

Neuronatin-mediated aberrant calcium signaling and endoplasmic reticulum stress - Neuronatin-mediated aberrant calcium signaling and endoplasmic reticulum stress

Sharma J, Mukherjee D, Rao SN, Iyengar S, Shankar SK, Satishchandra P, Jana NR. J Biol Chem. 2013 Mar 29;288(13):9482-90.

Neuronatin in a subset of glioblastoma multiforme tumor progenitor cells is - Neuronatin in a subset of glioblastoma multiforme tumor progenitor cells is

Xu DS, Yang C, Proescholdt M, Brundl E, Brawanski A, Fang X, Lee CS, Weil RJ, Zhuang Z, Lonser RR. PLoS One. 2012;7(5):e37811.

CELF4 regulates translation and local abundance of a vast set of mRNAs, including - CELF4 regulates translation and local abundance of a vast set of mRNAs, including

Wagnon JL, Briese M, Sun W, Mahaffey CL, Curk T, Rot G, Ule J, Frankel WN. PLoS Genet. 2012;8(11):e1003067.

Lafora disease ubiquitin ligase malin promotes proteasomal degradation of - Lafora disease ubiquitin ligase malin promotes proteasomal degradation of

Sharma J, Rao SN, Shankar SK, Satishchandra P, Jana NR. Neurobiol Dis. 2011 Oct;44(1):133-41.

Abundant expression of neuronatin in normal eccrine, apocrine and sebaceous - Abundant expression of neuronatin in normal eccrine, apocrine and sebaceous

Dugu L, Takahara M, Tsuji G, Iwashita Y, Liu X, Furue M. J Dermatol. 2010 Sep;37(9):846-8.

Neuronatin promotes neural lineage in ESCs via Ca(2+) signaling. - Neuronatin promotes neural lineage in ESCs via Ca(2+) signaling.

Lin HH, Bell E, Uwanogho D, Perfect LW, Noristani H, Bates TJ, Snetkov V, Price J, Sun YM. Stem Cells. 2010 Nov;28(11):1950-60.

miR-375 maintains normal pancreatic alpha- and beta-cell mass. - miR-375 maintains normal pancreatic alpha- and beta-cell mass.

Poy MN, Hausser J, Trajkovski M, Braun M, Collins S, Rorsman P, Zavolan M, Stoffel M. Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5813-8. doi:

Loss of neuronatin expression is associated with promoter hypermethylation in - Loss of neuronatin expression is associated with promoter hypermethylation in

Revill K, Dudley KJ, Clayton RN, McNicol AM, Farrell WE. Endocr Relat Cancer. 2009 Jun;16(2):537-48.