Anti-Neuroserpin antibody
| Name | Anti-Neuroserpin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab33077 |
| Prices | $370.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Rat Neuroserpin |
| Description | Rabbit Polyclonal |
| Gene | SERPINI1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Neuroserpin antibody (ab33077) at 1/250 dilutionLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab33077 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab33077, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab33077 staining Neuroserpin in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using a cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. The ab33077 produces a pretty cytoplasmic staining in a subpopulation of cortical neurons and some neurons in the brainstem.See Abreview
All lanes : Anti-Neuroserpin antibody (ab33077) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
Product References
A multilevel screening strategy defines a molecular fingerprint of - A multilevel screening strategy defines a molecular fingerprint of
Roet KC, Franssen EH, de Bree FM, Essing AH, Zijlstra SJ, Fagoe ND, Eggink HM, Eggers R, Smit AB, van Kesteren RE, Verhaagen J. J Neurosci. 2013 Jul 3;33(27):11116-35.
Neuroserpin protects neurons from ischemia-induced plasmin-mediated cell death - Neuroserpin protects neurons from ischemia-induced plasmin-mediated cell death
Wu J, Echeverry R, Guzman J, Yepes M. Am J Pathol. 2010 Nov;177(5):2576-84.