Anti-Neurotrophin 4 antibody [2E8]

• Supplier: Abcam
• Catalog: ab87394
• Prices: $384.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 2E8
• Applications: ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Human
• Antigen: Recombinant protein corresponding to amino acids 81-210 of human Neurotrophin 4 (NM_006179), expressed in E
• Description: Mouse Monoclonal
• Gene: NTF4
• Conjugate: Unconjugated

Product images

ab87394 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87394 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-Neurotrophin 4 antibody [2E8] (ab87394) at 1/5000 dilutionLane 1 : HEK293T cells transfected with the pCMV6-ENTRY control cDNA for 48 hrs and lysed.Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Neurotrophin 4 cDNA for 48 hrs and lysed.Lysates/proteins at 5 µg per lane.

Anti-Neurotrophin 4 antibody [2E8] (ab87394) at 1/5000 dilution + mature recombinant human NT4 (14 KDa)

Immunohistochemical analysis of paraffin embedded human breast tissue using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded adenocarcinoma of human breast tissue, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded human colon tissue, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded adenocarcinoma of human colon tissue, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded human kidney tissue, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded carcinoma of human kidney tissue, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded human liver tissue, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded carcinoma of human liver tissue, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded human lung tissue, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded carcinoma of human lung tissue, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded carcinoma of human pancreas, using ab87394 at a 1/50 dilution.

Immunohistochemical analysis of paraffin embedded human endometrium tissue, using ab87394 at a 1/50 dilution.