All lanes : Anti-Neurotrypsin antibody (ab59451) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from human teratocarcinoma Tera-1 cells (treated with TPA) at 30 µlLane 2 : Whole cell lysate prepared from human teratocarcinoma Tera-1 cells (treated with TPA) at 25 µlLane 3 : Whole cell lysate prepared from human teratocarcinoma Tera-1 cells (treated with TPA) at 20 µlLane 4 : Whole cell lysate prepared from human teratocarcinoma Tera-1 cells (treated with TPA) at 15 µlLane 5 : Whole cell lysate prepared from human teratocarcinoma Tera-1 cells (treated with TPA) at 10 µlLane 6 : Whole cell lysate prepared from human teratocarcinoma Tera-1 cells (treated with TPA) at 5 µl