Flow cytometric analysis of permeabilized Jurkat cells labeling NFAT2 (red) with ab177464 at 1/10 dilution, or a rabbit IgG (negative) (green).
Immunofluorescence analysis of Raji cells labeling NFAT2 (green) with ab177464 at 1/50 dilution. DAPI nuclear staining (blue).
All lanes : Anti-NFAT2 antibody [EPR2431(N)(B)] (ab177464) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : Raji cell lysateLane 3 : Ramos cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique