Anti-NFIA antibody
| Name | Anti-NFIA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab41851 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Chicken |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human NFIA |
| Description | Rabbit Polyclonal |
| Gene | NFIA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-NFIA antibody (ab41851) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 7 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 8 : Liver (Mouse) Mouse Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab41851 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41851, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Ab41851 staining NFIA in motorneurons of rat brain tissue sections by IHC-Fr. Sections were PFA fixed and incubated with ab41851, diluted 1/100, for 24 hours at 4°C. Chromeo™ 488 conjugated goat polyclonal to rabbit IgG ab60314, diluted 1/2000, was used as the secondary antibody.See Abreview
Product References
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The extracellular matrix molecule tenascin C modulates expression levels and - The extracellular matrix molecule tenascin C modulates expression levels and
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Impaired micro-RNA pathways diminish osteoclast differentiation and function. - Impaired micro-RNA pathways diminish osteoclast differentiation and function.
Sugatani T, Hruska KA. J Biol Chem. 2009 Feb 13;284(7):4667-78.