Lane 1 : Anti-Nicastrin antibody (ab45425) at 0.5 µg/mlLane 2 : Anti-Nicastrin antibody (ab45425) at 1 µg/mlLane 1 : Mouse brain tissue lysateLane 2 : Mouse brain tissue lysateLysates/proteins at 15 µg per lane.Secondaryanti-rabbit HRP at 1/10000 dilution
ab45425 at 2.5 µg/ml staining Nicastrin in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Immunofluorescence of Nicastrin in Human Brain cells using ab45425 at 20 ug/ml.
ab45425 staining Nicastrin in Human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2500 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.See Abreview