Anti-NKCC1 antibody

• Supplier: Abcam
• Catalog: ab59791
• Prices: $391.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F WB IHC-P IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide derived from the N terminal region of human NKCC1
• Description: Rabbit Polyclonal
• Gene: SLC12A2
• Conjugate: Unconjugated

Product images

ab59791 staining NKCC1 in Mouse salivary gland tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/600 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.See Abreview

ab59791 staining NKCC1 in Rat brain stem tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Trition and blocked with 5% BSA for 3 hours at 20°C; antigen retrieval was by heat mediation in a citrate sodium buffer. Samples were incubated with primary antibody (1/1000) for 15 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

IHC image of ab59791 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59791, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Nanoparticle-mediated gene silencing confers radioprotection to salivary glands - Nanoparticle-mediated gene silencing confers radioprotection to salivary glands

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NKCC1 and KCC2 protein expression is sexually dimorphic in the hippocampus and - NKCC1 and KCC2 protein expression is sexually dimorphic in the hippocampus and

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Decreased Immunoreactivities of the Chloride Transporters, KCC2 and NKCC1, in the - Decreased Immunoreactivities of the Chloride Transporters, KCC2 and NKCC1, in the

Suh MW, Ahn SC. Clin Exp Otorhinolaryngol. 2012 Sep;5(3):117-21.

Decreased Immunoreactivities and Functions of the Chloride Transporters, KCC2 and - Decreased Immunoreactivities and Functions of the Chloride Transporters, KCC2 and

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Acquisition of cisplatin-resistance in malignant mesothelioma cells abrogates - Acquisition of cisplatin-resistance in malignant mesothelioma cells abrogates

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