All lanes : Anti-NLRC3 antibody (ab77817) at 1 µg/mlLane 1 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.