Lanes 1 - 3 : Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/mlLane 4 : NR2B antibody (positive control)Lane 1 : mouse cortical extract with Mouse NMDAR2B (unmodified ) peptide (ab19637) at 2 µg/mlLane 2 : mouse cortical extract with Mouse NMDAR2B (phospho Y1252) peptide (ab19636) at 2 µg/mlLane 3 : mouse cortical extractLane 4 : mouse cortical extractLysates/proteins at 20 µg per lane.
IHC-FoFr image of NMDAR2B(pY1252) staining on mouse brain sections (A) Hypothalamic paraventricular nucleus and (B) Cerebral cortex using ab18532(1:100). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.See Abreview