Anti-NMDAR2B antibody [NR2B]
| Name | Anti-NMDAR2B antibody [NR2B] |
|---|---|
| Supplier | Abcam |
| Catalog | ab28373 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | NR2B |
| Applications | ICC/IF WB IP FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457 |
| Description | Mouse Monoclonal |
| Gene | GRIN2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/898_ab28372-2.png)
Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue
![Lanes 2 - 4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilutionLanes 5 - 7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilutionLane 2 : As aboveLane 3 : Mouse brain (50ug)Lane 4 : Rat brain (50ug)Lane 5 : As aboveLane 6 : Mouse brain (50ug)Lane 7 : Rat brain (50ug)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/899_ab28373_1.jpg)
Lanes 2 - 4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilutionLanes 5 - 7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilutionLane 2 : As aboveLane 3 : Mouse brain (50ug)Lane 4 : Rat brain (50ug)Lane 5 : As aboveLane 6 : Mouse brain (50ug)Lane 7 : Rat brain (50ug)
![Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/900_NMDAR2B-Primary-antibodies-ab28373-12.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.