Western blot analysis of immunoprecipitation pellet from MOLT4 cell lysate immunoprecipitated using ab181257 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling NME3 with ab181257 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
All lanes : Anti-NME3 antibody [EPR13117] (ab181257) at 1/2000 dilutionLane 1 : MOLT4 cell lysateLane 2 : Raji cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution