Anti-NNF1R antibody [EPR17298]

• Supplier: Abcam
• Catalog: ab199531
• Prices: $359.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17298
• Applications: IP WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant full length protein within Human NNF1R aa 1 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: PMF1-BGLAP
• Conjugate: Unconjugated

Product images

All lanes : Anti-NNF1R antibody [EPR17298] (ab199531) at 1/1000 dilutionLane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 3 : HEK293 (Human epithelial cells from embryonic kidney) whole cell lysateLane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

All lanes : Anti-NNF1R antibody [EPR17298] (ab199531) at 1/5000 dilutionLane 1 : Human fetal brain tissue lysateLane 2 : Human fetal kidney tissue lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilutiondeveloped using the ECL technique

All lanes : Anti-NNF1R antibody [EPR17298] (ab199531) at 1/1000 dilutionLane 1 : C6 (Rat glial tumor cells) whole cell lysateLane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 3 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

NNF1R was immunoprecipitated from 1mg of HeLa  (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179466 at 1/80 (or 5 μg).  Western blot was performed using ab199531 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell extract at 10ug (input)Lane 2: HeLa whole cell extract NNF1R immunoprecipitateLane 3: Rabbit monoclonal IgG (ab172730) instead of ab199531 in HeLa whole cell extract immunoprecipitation.Blocking and dilution buffer and concentration: 5% NFDM/TBST.