Anti-Noggin antibody
| Name | Anti-Noggin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab16054 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Human, Horse, Chicken, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Noggin |
| Description | Rabbit Polyclonal |
| Gene | NOG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Noggin antibody (ab16054) at 1 µg/mlLane 1 : Noggin Mouse Recombinant ProteinLane 2 : Noggin Mouse Recombinant Protein with Human Noggin peptide (ab16380) at 1 µg/mlLysates/proteins at 0.01 µg per lane.
Image courtesy of Human Protein Atlas ab16054 stainning Noggin in paraffin-embedded human liver tissue, showing a cytoplasmic and/or membranous distribution in both hepatocytes and bile duct cells. Paraffin embedded tissue was incubated with ab16054 (1/175 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab16054 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of Noggin stained primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16054, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h.. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.See Abreview
All lanes : Anti-Noggin antibody (ab16054) at 1 µg/mlLane 1 : Noggin Human Recombinant ProteinLane 2 : Noggin Mouse Recombinant ProteinLysates/proteins at 0.1 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-Noggin antibody (ab16054) at 1 µg/ml + Active human Noggin full length protein (ab73756) at 1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 4 minutes
Product References
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