Lane 1 : Anti-Nogo A antibody (ab62024) at 0.5 µg/mlLane 2 : Anti-Nogo A antibody (ab62024) at 1 µg/mlLane 1 : Human brain tissue lysateLane 2 : Human brain tissue lysateLysates/proteins at 15 µg per lane.Secondaryanti-rabbit IgG
ab62024, at 2.5µg/ml, staining Mouse Nogo A in brain tissue, by Immunohistochemistry
ICC/IF image of ab62024 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62024, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of NogoA in Mouse Brain cells using ab62024 at 20 ug/ml.