Anti-Nogo antibody [EPR12265]

• Supplier: Abcam
• Catalog: ab180847
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR12265
• Applications: WB ICC/IF ICC/IF IP FC IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Nogo aa 100-200 (Cysteine residue)
• Description: Rabbit Monoclonal
• Gene: RTN4
• Conjugate: Unconjugated

Product images

All lanes : Anti-Nogo antibody [EPR12265] (ab180847) at 1/1000 dilutionLane 1 : U87-MG cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human brain tissue labeling Nogo with ab180847 at a 1/50 dilution.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human breast carcinoma tissue labeling Nogo with ab180847 at a 1/50 dilution.

Immunofluorescence analysis of U87-MG cells labeling Nogo with ab180847 at a 1/100 dilution.

Flow cytometry analysis of permeabilized HeLa cells labeling Nogo (red) with ab180847 at a 1/10 dilution, or negative control rabbit IgG (green)

Western blot analysis on Immunoprecipitation pellet from either 1) HeLa cell lysate, or 2) 1xPBS (negative control); showing Nogo, using ab180847 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.