Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling NOLC1 with ab184550 at a 1/300 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
Immunofluorescence staining of -20℃ Acetone fixed K562 cells labeling NOLC1 using ab184550 at a 1/500 dilution (Red). Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution. Counterstained with Dapi (blue).
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling NOLC1 at a 1/100 dilution, counterstained with Hematoxylin.
All lanes : Anti-NOLC1 antibody [EPR14896] (ab184550) at 1/50000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution