Anti-NOM1 antibody [EPR17316-104]

• Supplier: Abcam
• Catalog: ab200352
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17316-104
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant fragment within Human NOM1 aa 50-350
• Description: Rabbit Monoclonal
• Gene: NOM1
• Conjugate: Unconjugated

Product images

All lanes : Anti-NOM1 antibody [EPR17316-104] (ab200352) at 1/100000 dilutionLane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLane 2 : HEK293 (Human embryonic kidney) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-NOM1 antibody [EPR17316-104] (ab200352) at 1/100000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-NOM1 antibody [EPR17316-104] (ab200352) at 1/1000 dilution + Human fetal brain lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling NOM1 with ab200352 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on K562 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab200352 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.