Anti-Notch1 antibody [A6]
| Name | Anti-Notch1 antibody [A6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab44986 |
| Prices | $384.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | A6 |
| Applications | ICC/IF ICC/IF FC IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant protein encoding the ligand binding region of human Notch1 |
| Description | Mouse Monoclonal |
| Gene | NOTCH1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab44986 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab44986, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Notch-1 mediated cardiac protection following embryonic and induced pluripotent - Notch-1 mediated cardiac protection following embryonic and induced pluripotent
Merino H, Singla DK. PLoS One. 2014 Jul 2;9(7):e101024.
Endocardial to myocardial notch-wnt-bmp axis regulates early heart valve - Endocardial to myocardial notch-wnt-bmp axis regulates early heart valve
Wang Y, Wu B, Chamberlain AA, Lui W, Koirala P, Susztak K, Klein D, Taylor V, Zhou B. PLoS One. 2013;8(4):e60244.
Regulation of PTEN/Akt pathway enhances cardiomyogenesis and attenuates adverse - Regulation of PTEN/Akt pathway enhances cardiomyogenesis and attenuates adverse
Yan B, Singla RD, Abdelli LS, Singal PK, Singla DK. PLoS One. 2013 Sep 24;8(9):e75580.
Differentiation therapy exerts antitumor effects on stem-like glioma cells. - Differentiation therapy exerts antitumor effects on stem-like glioma cells.
Campos B, Wan F, Farhadi M, Ernst A, Zeppernick F, Tagscherer KE, Ahmadi R, Lohr J, Dictus C, Gdynia G, Combs SE, Goidts V, Helmke BM, Eckstein V, Roth W, Beckhove P, Lichter P, Unterberg A, Radlwimmer B, Herold-Mende C. Clin Cancer Res. 2010 May 15;16(10):2715-28.
Mesenchymal progenitor cell markers in human articular cartilage: normal - Mesenchymal progenitor cell markers in human articular cartilage: normal
Grogan SP, Miyaki S, Asahara H, D'Lima DD, Lotz MK. Arthritis Res Ther. 2009;11(3):R85.
T-cell differentiation of multipotent hematopoietic cell line EML in the OP9-DL1 - T-cell differentiation of multipotent hematopoietic cell line EML in the OP9-DL1
Kutlesa S, Zayas J, Valle A, Levy RB, Jurecic R. Exp Hematol. 2009 Aug;37(8):909-23.
Itch genetically interacts with Notch1 in a mouse autoimmune disease model. - Itch genetically interacts with Notch1 in a mouse autoimmune disease model.
Matesic LE, Haines DC, Copeland NG, Jenkins NA. Hum Mol Genet. 2006 Dec 15;15(24):3485-97. Epub 2006 Nov 9.