Anti-NOX2/gp91phox antibody

• Supplier: Abcam
• Catalog: ab80508
• Prices: $359.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF FC IHC-F IHC-P WB
• Species Reactivities: Mouse, Rat, Human, Rabbit, Bovine, Dog
• Antigen: Synthetic peptide: KTIASQHPNT RIGVFLCGPE ALAETLSKQS ISNSESGPRG VHFIFNKENF , corresponding to C terminal amino acids 522-571 of Human NOX2/gp91phox (NP_000388) (Peptides available as ab111671)
• Blocking Peptide: Human NOX2/gp91phox peptide
• Description: Rabbit Polyclonal
• Gene: CYBB
• Conjugate: Unconjugated

Product images

Anti-NOX2/gp91phox antibody (ab80508) at 1 µg/ml + MCF7 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

All lanes : Anti-NOX2/gp91phox antibody (ab80508) at 1/1000 dilutionLane 1 : Mouse liver whole cell lysateLane 2 : Mouse liver whole cell lysateLysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to rabbit IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under non-reducing conditions.

ICC/IF image of ab80508 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80508, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab80508 staining NOX2/gp91phox in Human monocyte-derivive dendritic cells by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with methanol. The sample was incubated with the primary antibody (1/200) for 30 minutes. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/300) was used as the secondary antibodySee Abreview

ab80508 staining NOX2/gp91phox in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% milk for 30 minutes at 37°C; antigen retrieval was by heat mediation with a Tris Borate EDTA buffer (pH 8.4). Samples were incubated with primary antibody (1/200 in antibody diluent) for 1 hour at 37°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/700) was used as the secondary antibody.See Abreview

Product References

Oxidative stress exaggerates skeletal muscle contraction-evoked reflex - Oxidative stress exaggerates skeletal muscle contraction-evoked reflex

Koba S, Watanabe R, Kano N, Watanabe T. Am J Physiol Heart Circ Physiol. 2013 Jan 1;304(1):H142-53. doi:

Angiotensin-converting enzyme 2 priming enhances the function of endothelial - Angiotensin-converting enzyme 2 priming enhances the function of endothelial

Chen J, Xiao X, Chen S, Zhang C, Chen J, Yi D, Shenoy V, Raizada MK, Zhao B, Chen Y. Hypertension. 2013 Mar;61(3):681-9.

Cellular and temporal expression of NADPH oxidase (NOX) isotypes after brain - Cellular and temporal expression of NADPH oxidase (NOX) isotypes after brain

Cooney SJ, Bermudez-Sabogal SL, Byrnes KR. J Neuroinflammation. 2013 Dec 17;10:155.

Antibody-induced nonapoptotic cell death in human lymphoma and leukemia cells is - Antibody-induced nonapoptotic cell death in human lymphoma and leukemia cells is

Honeychurch J, Alduaij W, Azizyan M, Cheadle EJ, Pelicano H, Ivanov A, Huang P, Cragg MS, Illidge TM. Blood. 2012 Apr 12;119(15):3523-33.

The voltage-gated proton channel Hv1 enhances brain damage from ischemic stroke. - The voltage-gated proton channel Hv1 enhances brain damage from ischemic stroke.

Wu LJ, Wu G, Akhavan Sharif MR, Baker A, Jia Y, Fahey FH, Luo HR, Feener EP, Clapham DE. Nat Neurosci. 2012 Mar 4;15(4):565-73.

Oxidized low-density lipoprotein induces secretion of interleukin-1beta by - Oxidized low-density lipoprotein induces secretion of interleukin-1beta by

Jiang Y, Wang M, Huang K, Zhang Z, Shao N, Zhang Y, Wang W, Wang S. Biochem Biophys Res Commun. 2012 Aug 24;425(2):121-6. doi:

Studies of mitochondrial and nonmitochondrial sources implicate nicotinamide - Studies of mitochondrial and nonmitochondrial sources implicate nicotinamide

Sakellariou GK, Vasilaki A, Palomero J, Kayani A, Zibrik L, McArdle A, Jackson MJ. Antioxid Redox Signal. 2013 Feb 20;18(6):603-21.

Aldosterone stimulates superoxide production in macula densa cells. - Aldosterone stimulates superoxide production in macula densa cells.

Zhu X, Manning RD Jr, Lu D, Gomez-Sanchez CE, Fu Y, Juncos LA, Liu R. Am J Physiol Renal Physiol. 2011 Sep;301(3):F529-35. doi:

Neuroprotection from retinal ischemia/reperfusion injury by NOX2 NADPH oxidase - Neuroprotection from retinal ischemia/reperfusion injury by NOX2 NADPH oxidase

Yokota H, Narayanan SP, Zhang W, Liu H, Rojas M, Xu Z, Lemtalsi T, Nagaoka T, Yoshida A, Brooks SE, Caldwell RW, Caldwell RB. Invest Ophthalmol Vis Sci. 2011 Oct 11;52(11):8123-31.

Leptin inhibits the proliferation of vascular smooth muscle cells induced by - Leptin inhibits the proliferation of vascular smooth muscle cells induced by

Rodriguez A, Gomez-Ambrosi J, Catalan V, Fortuno A, Fruhbeck G. Mediators Inflamm. 2010;2010:105489.