Anti-NOX2/gp91phox antibody [54.1]
| Name | Anti-NOX2/gp91phox antibody [54.1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab80897 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 54.1 |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Tissue/ cell preparation (Human): Partially Purified human neutrophil flavocytochrome b (heparin Ultrogel/lectin affinity purification in Triton X-100) |
| Description | Mouse Monoclonal |
| Gene | CYBB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-NOX2/gp91phox antibody [54.1] (ab80897) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/1555_NOX2gp91phox-Primary-antibodies-ab80897-2.jpg)
All lanes : Anti-NOX2/gp91phox antibody [54.1] (ab80897) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab80897 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80897, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab80897 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Nox1 oxidase suppresses influenza a virus-induced lung inflammation and oxidative - Nox1 oxidase suppresses influenza a virus-induced lung inflammation and oxidative
Selemidis S, Seow HJ, Broughton BR, Vinh A, Bozinovski S, Sobey CG, Drummond GR, Vlahos R. PLoS One. 2013;8(4):e60792.
Evaluation of two anti-gp91phox antibodies as immunoprobes for Nox family - Evaluation of two anti-gp91phox antibodies as immunoprobes for Nox family
Baniulis D, Nakano Y, Nauseef WM, Banfi B, Cheng G, Lambeth DJ, Burritt JB, Taylor RM, Jesaitis AJ. Biochim Biophys Acta. 2005 Sep 25;1752(2):186-96.
Topological mapping of neutrophil cytochrome b epitopes with phage-display - Topological mapping of neutrophil cytochrome b epitopes with phage-display
Burritt JB, Quinn MT, Jutila MA, Bond CW, Jesaitis AJ. J Biol Chem. 1995 Jul 14;270(28):16974-80.