All lanes : Anti-NQO2 antibody [EPR13345] (ab181049) at 1/1000 dilutionLane 1 : Mouse kidney lysateLane 2 : Rat heart lysateLane 3 : Rat kidney lysateLane 4 : Rat spleen lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometric analysis of C6 cells labeling NQO2 with ab181049 at 1/140 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
All lanes : Anti-NQO2 antibody [EPR13345] (ab181049) at 1/5000 dilutionLane 1 : L6 cell lysateLane 2 : C2C12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution