
All lanes : Anti-NR2F2 antibody (ab42672) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Rat Colon Tissue LysateLane 3 : Rat Thymus Tissue LysateLane 4 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab3950)Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.