Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, centrosome and cell junctions. Recommended concentration of ab122427 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122427, at a 1/200 dilution, staining NRAP in paraffin embedded Human liver tissue by Immunohistochemistry.
Anti-NRAP antibody (ab122427) at 1/1000 dilution + Sheep cardiac tissue at 10 µgSecondaryDonkey Anti-Rabbit IgG (H+L) at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 190 kDa (why is the actual band size different from the predicted?)Exposure time : 50 secondsThis image is courtesy of an anonymous abreview.See Abreview