All lanes : Anti-NRF1 antibody [EPR5554(N)] (ab175932) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : Hela cell lysateLane 3 : Human fetal heart cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.
Immunofluorescent staining of HeLa cells labeling NRF1 with ab175932 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling NRF1 at 1/10 dilution.
Flow cytometric analysis of permeabilized 293T cells labeling NRF1 with ab175932 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling NRF1 with ab175932 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling NRF1 with ab174932 at 1/50 dilution.