Anti-NSE antibody
| Name | Anti-NSE antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53025 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F FC ICC/IF ICC/IF ELISA WB IHC-P |
| Species Reactivities | Mouse, Rat, Human, Pig |
| Antigen | Synthetic peptide derived from human NSE |
| Description | Rabbit Polyclonal |
| Gene | ENO2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab53025 staining NSE in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3 Triton-X, and blocked with 5% serum for 1 hour at room temperature. The sample was incubated with primary antibody (1/250) at 4°C for 16 hours. Ab96921, DyLight® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53025 at 1/50-1/100 dilution. Left: without immunizing peptide; Right: with immunizing peptide
All lanes : Anti-NSE antibody (ab53025) at 1/500 dilutionLane 1 : Extracts from HepG2 cells with no immunizing peptideLane 2 : Extracts from HepG2 cells with immunizing peptide
ICC/IF image of ab53025 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53025, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab53025 staining NSE in mouse brain tissue section by Immunohistochemistry (PFA-perfussion fixed frozen tissue sections). Tissues were collected from mice in each group at 10 weeks. Mice were euthanized, the brains were exposed and removed from the body. Then the brain was cut, on ice and fixed in 4% polyformaldehyde or glutaraldehyde solution for histological examination. The primary antibody was used at 1/75 dilution in PBS and incubated with sample for 2 hours at 37°C. A HRP-conjugated secondary antibody was used at for 1hr at 37°C in a humidity box.
Intracellular flow analysis of NSE on N2A mouse neuroblastoma cells using ab53025 at 1:500 dilution. Purple histogram represents rabbit IgG negative control; green line represents anti-NSE antibody (ab53025).See Abreview
Product References
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