Anti-NSE antibody [5A4]
| Name | Anti-NSE antibody [5A4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab10169 |
| Prices | $379.00 |
| Sizes | 250 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 5A4 |
| Applications | IHC-P ELISA WB ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | ENO2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab10169 staining human normal pancreas. Staining is localized to cell membrane and cytoplasm.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Evidence that a panel of neurodegeneration biomarkers predicts vasospasm, - Evidence that a panel of neurodegeneration biomarkers predicts vasospasm,
Siman R, Giovannone N, Toraskar N, Frangos S, Stein SC, Levine JM, Kumar MA. PLoS One. 2011;6(12):e28938.
Ionizing radiation induces prostate cancer neuroendocrine differentiation through - Ionizing radiation induces prostate cancer neuroendocrine differentiation through
Deng X, Liu H, Huang J, Cheng L, Keller ET, Parsons SJ, Hu CD. Cancer Res. 2008 Dec 1;68(23):9663-70.